动物造模 z-bio 2021-07-12 695

　　Objective: To observe the inducing effect of temozolomide on the programmed death of rat glioma C6 cells and its possible molecular mechanism.

　　Method: Observe different concentrations of temozolomide at different times to treat rat glioma. Rat brain glioma C6 cell line, the inhibition rate was observed by MTT method, and the best time and concentration were selected. The cells were treated with 400 μg/ml temozolomide for 24 hours, and the induction effect of temozolomide on the programmed death of rat glioma C6 cell line was observed by HE staining, hexist, Western blot, tunneling and immunohistochemistry.

　　Results: MTT detection, HE staining, hochest and tunnel results show that temozolomide has an apoptosis-inducing effect on rat glioma C6 cells. The results of immunohistochemistry and Western blot showed that the expression of pro-apoptotic protein Bax was up-regulated, and the expression of apoptosis-inhibiting protein Bcl-2 was down-regulated. No changes in the expression of major caspase proteins were observed.

　　Conclusion: Temozolomide has an apoptosis-inducing effect on glioma C6 cells, which may be achieved through a caspase-independent approach.