动物造模 z-bio 2021-07-12 474

　　Objective: To investigate the effect of alcohol exposure during pregnancy on the basic rhythmic respiratory discharge activity (RRDA) of the medulla oblongata slices of newborn rats.

　　Method: Prepare brain slice specimens of newborn rats containing the medial hypoglossal nerve root (mNRF), hold the hypoglossal nerve root, and hold the perfused modified Kreb solution (MKS) medulla oblongata brain slice with an adsorption electrode On the ventral surface, the RDA and RDA of the hypoglossal nerve were recorded. The experiment is divided into 5 groups: control group, 4? sperm exposure group, 6? sperm exposure group, 8? sperm exposure group, to study the influence of different concentrations of alcohol exposure during pregnancy on RRDA. And 10? fine exposure group.

　　Results: In the control group within 50 minutes, there was no statistical difference in inhalation time (TI), respiratory frequency (RF), discharge integral amplitude (IA), 4? sperm exposure group, 6? sperm exposure group, 8? sperm exposure group (RRDA) . The RDA rhythm of the 10 refined exposure group was irregular. Compared with the control group, the RRDA of the 4? 0? sperm exposure group gradually decreased with the increase of alcohol concentration, and the TI shortened. F drops and IA weakens.

　　Conclusion: Alcohol exposure during pregnancy inhibits the RRDA of the hypoglossal nerve root in the medulla oblongata of the offspring of newborn rats. This may be the basis of impaired respiratory function in the offspring of pregnancy.