动物造模 z-bio 2021-07-13 386

　　Objective: To study the subacute inhalation toxicity of ivermectin to rats and find the maximum unaffected dose.

　　Method: Use SPF-grade Sprague-Dawley (SD) rats, a total of 72 rats, randomly divided into 6 groups. There are 12 rats in each group, half male and half male. Set up three dose groups of 190, 380, 750 mg/m3 and a solvent control group (0.03?ween-80 solution), a blank control group and a supplementary group (750 mg/m3). Using the motivational (oral and nasal) inhalation method, animals are exposed to toxic substances once a day for 4 hours, 5 days a week, up to 28 days. After stopping the poisoning, another animal group was observed for 14 days. After the experiment, the animal's blood routine, biochemistry, body weight and organ coefficients were tested, and histopathological examination was performed.

　　Results: Both male and female rats in the 750 mg/m3 group showed toxic reactions such as fluffy skin, turbidity, salivation, and tremor in the later period of exposure. In the 750 mg/m3 administration group, the food utilization rate of female rats decreased, the serum alanine aminotransferase (ALT) level increased (Pu003c0.01), and the liver organ coefficient (visceral ratio) increased (Pu003c0.. 05) Histopathological examination revealed that some rat liver cells were turbid and swollen. Male rats in the 750 mg/m3 group lost body weight at the 4th week, and serum urea nitrogen (BUN) and ALT levels increased (Pu003c0.01). And the total cholesterol (CHOL) level is reduced (Pu003c0.05).

　　Conclusion: The maximum unaffected dose of ivermectin in the subacute inhalation toxicity study in rats is 380 mg/m3 (4 h/d), male and female.