动物造模 z-bio 2021-07-13 299

　　OBJECTIVE: To investigate the effect and mechanism of changes in EEG activity in the submarginal cortex (IL) telemetry on drug-seeking behavior in morphine-dependent rats during the sinking process.

　　Method: SD rats were randomly divided into model group and control group, with stereotactic electrodes embedded in the IL area. The rat model group was successively created into a morphine dependence model and a regression model, and a wireless telemetry system was used. Record the IL during the return period. Changes in the local electrical activity of the brain.

　　Results: The staying time of the model group rats in the white box was significantly longer than before the injection of morphine and the control group rats 1-2 days after the drug withdrawal; 1-2 days after sinking training, the white box staying time of the model group rats was significantly longer Shorter than morphine. Before injection, there was no significant difference from the control group. Compared with the control group, the model group stayed in the white box for 2 days after stopping the drug, the EEGβ wave in the IL area was significantly increased, and the delta wave was significantly reduced. The shuttle from the black box to the white box resulted in a significant increase in delta waves, while a significant decrease in alpha and beta waves. When the rats in the model group stayed in the white box, the θ wave in the IL area was significantly lower than that in the control group. Compared with the withdrawal period, the beta wave and theta wave were significantly reduced. Compared with the control group, there was no significant difference in the IL EEG of the shuttle from black box to white box, but compared with the withdrawal period, the delta wave was significantly reduced, and the α wave and β wave were significantly increased.

　　Conclusion: The abnormal changes in the brain electrical activity of IL area in morphine-dependent rats during the drug search sinking process may affect the formation of drug search motivation, thereby inhibiting the development and maintenance of drug search behavior.