慢性间歇性缺氧 z-bio 2021-07-13 789

　　Objective: To investigate the effects of inflammatory factors caused by chronic intermittent hypoxia and reoxygenation on glucose metabolism and skeletal muscle glucose transporter 4 (GLUT-4) expression in rats.

　　Methods: 24 SD male rats were divided into blank group (UC group), chronic intermittent hypoxia group (CIH group) and reoxygenation group (RH group). After the model was established, all rats were oxidized. Use Enzyme peroxidase, radioimmunoassay and ELISA detect changes in blood glucose, serum insulin and inflammatory factors. Western blotting detects the expression of skeletal muscle GLUT-4 protein.

　　Results: Rat fasting blood glucose, CIH group was higher than UC and RH group (Pu003c0.05), RH group was higher than UC group (Pu003c0.05); serum insulin and insulin resistance index, CIH group was higher than UC group and RH group ( Pu003c0.05). The serum inflammatory indexes TNF-α and IL-6 of rats in the CIH group were significantly higher than those in the UC group and the RH group (Pu003c0.05), and the RH group was higher than the UC group (Pu003c0.05). ). Rat skeletal muscle GLUT4 protein, CIH group was significantly lower than UC and RH group (Pu003c0.05), RH group was lower than UC group (Pu003c0.05).

　　Conclusion: Chronic intermittent hypoxia can lead to increased inflammatory factors and insulin resistance in rats. Insulin resistance in rats is related to the decrease of skeletal muscle GLUT-4 protein caused by inflammatory factors.