动物造模 z-bio 2022-02-01 935

　　Objective: To observe the effects of DEHP exposure on the growth and development of mouse placenta, the number of uNK cells at the maternal-fetal interface, and angiogenesis in vivo through exposure to bis(2-ethylhexyl) phthalate (DEHP) during pregnancy.

　　Method: From the first day of pregnancy, through forced gastric tube feeding outside the body, various doses of DEHP125, 250, 500mg?kg-1?D-1 were given, and uterus and placenta specimens were taken and weighed. .. On the 13th day of pregnancy, HE staining and immunohistochemical methods were used to observe the growth and development of the mouse placenta, the effect on the number of uNK cells at the maternal-fetal interface and angiogenesis.

　　Results: (1) Compared with the control group, low-dose DEHP125, 250 mg?kg-1?D-1 had no significant effect on the number of embryo implantation (P→0.05). However, the high-dose group of 500 mg?Kg-1?D-1 DEHP may significantly reduce the number of embryo implantation (Pu003c0.05). In addition, DEHP exposure can cause a significant increase in the rate of fetal miscarriage. (2) Compared with the control group (0.0786±0.0143 g), DEHP reduced placental weight in a dose-dependent manner of 125 and 250. , 500mg?kg-1?D-1 (each: 0.0637±0.0133, 0.0587±0.0176, 0.0524±0.0183g; Pu003c0.01), significantly reducing the total area of the placenta and the area of the trophoblast. Stratified cells; (3) DEHP Exposure significantly reduced the number of fetal blood vessel branches and vascular collapse and closure; (4) Compared with the control group (105.1±14.2)/HP), DEHP exposure inhibited the number of fetal uNK cells in a dose-dependent manner (83.2±14.2, respectively) 10.3, 60.7±12.4), 50.4±14.5/HP; Pu003c0.01).

　　Conclusion: Exposure to DEHP during pregnancy can significantly damage the growth and development of the placenta, probably by affecting the formation of blood vessels and the number of uNK cells at the maternal-fetal interface.