动物造模,大鼠急性肝损伤 z-bio 2021-07-13 330

　　Objective: To study the protective effect of Nrf2 oxidative damage pathway on CCl4-induced acute liver injury in rats.

　　Methods: Twenty male Wistar rats were randomly divided into solvent control group and CCl4 group, each group selected 10 male Wistar rats and 10 male Wistar rats, selected the male pronucleus of transgenic rats, and realized the microinjection through the operator . The rf2-tk integration and specific expressions are used as the CCl4 + rf2 integration group. The solvent control group received 1? Sorbate 80 intravenously for 4 days, and the CCl4 and Nrf2-tk combined group received 1? Sorbate 80 intravenously for 4 days. On the 4th day, 1? Sorbate was injected intravenously for 80 minutes. The rats were given 7.5 mg/kg CCl4 intravenously, and the rats were sacrificed 24 hours later. Determine serum AST, ALT, LDH levels, determine liver tissue MDA, GSH, GSSG content, and calculate GSH/GSSG ratio. Collect liver tissue, usually prepare paraffin-embedded sections, HE stain, and observe the pathological changes of liver tissue under light microscope.

　　Result: Compared with the solvent control group, the serum AST, ALT and LDH levels of rats in the CCl4 group were significantly increased (P\u003c0.05). The content of liver MDA and the ratio of GSH/GSSG indicate that the Nrf2-tk integrated group can effectively reduce the lipid peroxidation damage and glutathione consumption caused by CCl4. Pathological observation of the liver showed that the Nrf2-tk integration group was compared with CCl4. group. Obviously, it reduces the damage caused by CCl4.

　　Conclusion: The Nrf2 anti-oxidative damage pathway has an important protective effect in the acute liver injury caused by carbon tetrachloride in rats.