动物造模 z-bio 2021-09-24 488

　　Objective: To observe the effect of negative pressure sealing drainage combined with heparin solution washing combined with seawater immersion on the healing of back burns in rabbits.

　　Method: Choose 20 New Zealand white rabbits, establish 4 1?BSA deep 2nd degree burn models on both sides of the spine, traditional dressing change (group A), regular dressing change after sea water immersion (group A) group B). ), closed negative pressure suction treatment after seawater immersion (group C), closed negative pressure suction combined with heparin cleaning after seawater immersion (group D). The wound exudation and necrosis were observed on the 1, 3, 5, and 7 days after the burn, and the materials were collected for HE and immunohistochemical staining. Observe the pathological changes of the wound and the expression of vascular endothelial growth factor (VEGF) and cd31 under a light microscope, and calculate the blood vessel density of the tissue. Observe the morphological changes of wound capillaries and endothelial cells under an electron microscope. Calculate the moisture content of the paper towel using the dry and wet weight method. Use millimeter graph paper on days 3, 5, 7 and 14 to calculate the wound healing rate.

　　Result: Compared with the unsoaked group (group A), the wounds after seawater immersion (group B) were dry, deepened and had a higher degree of edema. Compared with group B, the wound necrosis tissue loss in group C and group D was faster, the basic blood supply was better, and the initial wound necrosis rate was lower than that in group B. After the burn, the water content of the wound tissue in each group increased, reaching a peak on the 3rd day, and the water content of the wound in group B was highest at each time point. The water content of groups C and D was lower than that of group B, and the difference was statistically significant. Observed by light microscope and electron microscope, the wounds in each group had inflammatory cell infiltration and varying degrees of vascular basement membrane damage after burns. Group B wounds had severe vascular endothelial cell damage, and contracted red blood cells contracted and accumulated significantly. Although cells and vascular embolism were found in the blood vessels, the capillary basement membrane damage in group D was significantly lighter, and the endothelial cell membrane was relatively intact. Scattered angiogenesis began to appear in the wounds of each group 3 days after injury, with the earliest in group D and the highest peak value. The microvessels in group B were formed later and had lower density.

　　Conclusion: Negative pressure closed drainage therapy combined with heparin solution wet pressure therapy can effectively reduce the degree of tissue edema after burns soaked in seawater, improve tissue microcirculation, accelerate the removal of necrotic tissue, and promote the growth of new blood vessels. An effective method for burns and burns after soaking.