胆管结扎,NOX4蛋白表达 z-bio 2021-07-14 434

　　OBJECTIVE: To design and establish a rat liver fibrosis model with biliary tract ligation and recanalization to observe the effect of biliary tract ligation and recanalization on the epithelial mesenchymal phenotype and the expression of oxidative stress-related proteins in rat liver tissue cells.

　　Method: 12 male Wistarats were randomly divided into sham operation group, bile duct ligation for 2 weeks, bile duct ligation for 4 weeks, bile duct ligation for 2 weeks, then bile duct ligation for 2 weeks, tissue evaluation... HE staining, Masson staining, etc. The degree of liver fibrosis in model rats; the expression of epithelial and mesenchymal marker proteins and oxidative stress-related proteins were detected by immunohistochemistry and western blotting.

　　Results: Compared with the sham operation group, prolonging the ligation time of the bile duct significantly increased the liver fibrosis in the model group, and the expression of α-SMA, type I collagen, NOX4, and vimentin increased significantly. After E-cadherin expression was ligated, the liver cirrhosis of rats in the recanalization group was significantly lower than that in the simple bile duct ligation group for 4 weeks. The OX4 and mesenchymal marker protein table is significantly lower than the simple table. The expression of endothelial cell marker protein was significantly higher in the 4-week ligation group and the 4-week ligation group.

　　Conclusion: Bile duct ligation can up-regulate the expression of interstitial phenotype-related protein and NOX4 protein in the rat liver, while inhibiting the expression of epithelial phenotype-related protein; after recanalization, the expression of intrahepatic epithelial surface-related protein in the original bile increases duct ligation Rats, but the expression of mesenchymal phenotype-related proteins and OX4 protein was significantly reduced.