动物造模,肺成纤维细胞 z-bio 2021-07-14 993

　　Objective: To investigate the effects of IL-27 and its receptor (WSX-1) on the proliferation, transformation and collagen synthesis of mouse lung fibroblasts induced by TGF-β1.

　　Method: Construct IL-27R/pCDNA3.1 overexpression vector. The experiment is divided into 6 groups: normal group, TGF-β1 group, IL-27 group, IL-27 receptor group, IL-27+IL-27 receptor group, TGF-β1+IL-27+IL-27 receptor Group body group. The cell proliferation was observed under a light microscope, and the MTT method was used to detect the effect of each treatment group on the proliferation of lung fibroblasts. T-PCR and Western blotting were used to detect the expression of myofibroblast marker a-SMA and type I and type III collagen. Immunofluorescence method was used to locate a-SMA protein and detect the expression.

　　Results: (1) IL-27 and IL-27R can inhibit the proliferation of lung fibroblasts; (2) TGF-β1 promotes the expression of α-SMA in lung fibroblasts and the lung promotes the synthesis of type I and type III collagen of fibroblasts; (3) IL-27 and its receptor inhibit the expression of α-SMA in lung fibroblasts and type I lung fibroblasts, and may reduce the synthesis of type III collagen. Effects on the proliferation and transformation of lung fibroblasts.

　　Conclusion: IL-27 or IL-27 receptor can inhibit the proliferation, transformation, and collagen synthesis of mouse lung fibroblasts induced by TGF-β1, but IL-27 and IL-27 receptors coexist, but they cannot exert an inhibitory effect. This may be due to the neutralization of exogenous IL-27 and IL-27 receptors that cannot activate cell-related signals.