动物造模,维生素C,骨髓间充质干细胞 z-bio 2021-10-08 750

　　Objective: To observe whether vitamin C (Vc) can promote the proliferation of bone marrow mesenchymal stem cells (BMMSC) in the elderly by increasing telomerase activity.

　　Method: Using bone-specific premature aging mice (SAMP6) as the experimental group, and R strain non-progeria mice (SAMR1) as the control group, the skeletal aging phenotype of SAMP6 mice was verified by microCT. Scanning technology. The BMMSCs of two groups of mice were separated and cultured, and BMMSCs of SAMP6 mice were treated with different concentrations of Vc. MTT method was used to detect the proliferation ability of BMMSC, draw growth curve, telomerase kit to detect telomerase activity, PCR and Western blotting to detect the expression level of telomerase reverse transcriptase (TERT). .

　　Result: SAMP6 mice have a bone aging phenotype. The proliferation ability and telomerase activity of BMMSC derived from SAMP6 mice were lower than those of BMMSC derived from SAMR1 mice, and the difference was statistically significant (Pu003c0.05). After the addition of Vc, the proliferation ability of SAMP6 mouse-derived BMMSC in a specific concentration range increased significantly with the increase of Vc concentration (Pu003c0.05), and its telomerase activity and TERT expression levels also increased (Pu003c0. 05). It is related to proliferation ability. Among them, the best Vc concentration for promotion is 100 μg/mL, and 1000 μg/mL has inhibitory effect.

　　Conclusion: Vitamin C may promote the growth of BMMSC in the elderly, and this effect may be achieved by increasing the activity of telomerase.