动物造模,裸鼠前列腺癌 z-bio 2021-07-14 754

　　OBJECTIVE: To study the effect of solanine on the growth of transplanted prostate cancer cells Du145 in nude mice and its molecular mechanism.

　　Method: In vivo experiments were conducted with the highly malignant metastatic prostate cancer cell line Du145 as an animal model. Du145 cells were subcutaneously inoculated to nude mice to establish a nude mouse subcutaneous tumor model. One week later, the inoculated nude mice were randomly divided into two groups: solanine experimental group and normal saline blank control group. 0.2 mL of solanine (50 μg/mL) and normal saline were used for solid tumors. The tumor was observed by injection every 3 days. The center grows in nude mice. Three weeks later, the nude mice were sacrificed by cervical dislocation. The tumor tissue was stripped, weighed, and the tumor inhibition rate was calculated based on the weight. Tumor. Real-time fluorescence quantitative PCR and Western blotting were used to detect the expression of cell cycle-related genes mRNA and protein in tumor cells of nude mice in each group. Tunnel in situ detection of tumor tissue apoptosis in nude mice in each group.

　　Result: The tumor formation rate of nude mice in the solanine group was significantly reduced, and the tumor growth rate of nude mice was significantly lower than that of the control group (Pu003c0.01). Solanin can inhibit the expression of CyclinD1, CyclinE1, CDK2, CDK4 and CDK6 gene mRNA and protein in tumor cells, and significantly increase the expression of p21 gene mRNA and protein. u003c0.01).

　　Conclusion: Solanum can regulate cell cycle G1/S checkpoints, promote cell apoptosis, and inhibit the growth of transplanted prostate cancer cells in nude mice.