动物造模,小鼠迟发性抑郁 z-bio 2021-10-08 859

　　Objective: To study the effect of continuous long-term rapid eye movement sleep deprivation (5 days) on depression-like behavior and amygdala monoamine oxidase A level in C57BL/6J mice.

　　Method: Adult male C57BL/6J mice were randomly divided into blank group, control group and REM sleep deprivation group, and a multi-platform water environment method was used to establish a REM sleep deprivation model. After sleep deprivation, the sports performance of each group at different time was tested by field test, and the depression-like behavior of each group was tested by forced swimming and sugar water preference test. The brains were then harvested to isolate the amygdala, and Western blot and real-time PCR were used to detect the expression level of monoamine oxidase A in each group.

　　Result: Within 1-3 days of EM sleep deprivation, the exercise performance of sleep-deprived mice decreased significantly, and returned to normal levels on the 4th day. In the forced swimming experiment on days 7-14, the mice were stretched. The preference for sugar water has also dropped significantly. Western blot and real-time PCR showed a significant increase in the expression level of monoamine oxidase A in the amygdala.

　　Conclusion: Continuous sleep deprivation and long-term rapid eye movements can cause delays in depression-like behavior in mice. This phenomenon may be related to changes in the level of amigdara monoamine oxidase.