动物造模,神经干细胞移植,脊髓损伤 z-bio 2021-07-15 259

　　Objective: To investigate the effect of bcl-2 gene-modified neural stem cell transplantation on the recovery of nerve function in rats with spinal cord injury.

　　Method: Culture rat neural stem cells in vitro, and use Ad-EGFP as a carrier to mediate B lymphoblastoma-2 gene (bcl-2) gene transfection into neural stem cells. These are divided into three groups: the control group and the negative transfection group, and the Bcl-2 transfection group. Western blot was used to detect the expression of bcl-2 protein in neural stem cells before and after transfection. 85 adult female SD rats (of which 72 were successfully modeled) were randomly divided into control group, NSC group, bcl-2-NSC group, and 24 rats/group. The rat model of acute spinal cord injury was established according to the modification. Done. Allen strike law. Evaluate motor function through BBB score and tilt board test. Seven days after modeling, RT-PCR and Western blotting were used to detect the expression of HSP27 and c-fos genes around the spinal cord injury area, and the TUNEL method was used to detect cell apoptosis. Four weeks after modeling, the specimens were taken for pathological section HE staining and fluorescence microscope observation to observe the survival and distribution of EGFP-labeled NSC, and SEP and MEP to observe the neuroelectrophysiological recovery of rats.

　　Results: After the bcl-2 gene was transfected into rat neural stem cells, the bcl-2 gene and protein expression levels of the bcl-2 transfection group were compared with the control group and the negative transfection group () PNSC group → control difference group and group Significant (Pu003c0.05).

　　The u003cSCS group has significant differences between the u003c control group and the u003c control group (Conclusion: Ad-EGFP gene (bcl-2) gene transfection is used as a vector to mediate type B lymphoma-2, so that neural stem cells can promote rat neural stem cells cultured in vitro Transplantation of proliferating Bcl-2 gene-modified neural stem cells can promote and increase the regeneration of nerve synapses in rats with spinal cord injury, reduce the expression of HSP27 in the spinal cord injury area, the expression of bcl-2 gene and the nerve cell transfection in the spinal cord injury area, and improve Movement and electrophysiological functions of rats' limbs.