动物造模 z-bio 2021-07-15 265

　　OBJECTIVE: To observe the effects of high-fat diet on the structure of hypothalamus, pituitary gland, testis and sex hormone levels in adolescent male rats, and to explore the effects of 6-week free-spirited exercise.

　　Method: 40 male SD rats weaned for the first time at the age of 3 weeks were divided into a normal diet control group (C group), a normal diet exercise group (CE group), a high-fat diet group (D group), and a high-fat diet group. Randomly divided. Fat diet exercise group (DE group). The C and D groups were fed quietly for 8 weeks, and the CE and ED groups were free to exercise for 6 weeks after 2 weeks of adaptive feeding, 1 hour a day, 5 days in the afternoon. After 8 weeks, blood was collected from the abdominal aorta of the rat, serum T, E2, FSH, LH were measured, testicular tissue homogenate was prepared, T, E2, and hypothalamic, pituitary, and testicular tissue sections were prepared. Then observe the following structural changes. Optical microscope.

　　Results: (1) Compared with group C, the detection rate of serum T and E2 in group D decreased, and the level of T in testicular tissue was significantly decreased (Pu003c0.05), but serum E2., FSH, and LH were significantly increased (Pu003c0 .05); vacuolar lipid droplets appear in the hypothalamus, pituitary eosinophils and basal cells are reduced, the area of seminiferous tubules, the proportion of sperm cells, and the number of interstitial cells are reduced, and the testis is reduced. (2) The levels of serum T-detection and testicular T-detection of rats in DE group were higher than those in group D, but serum E2, FSH, and LH were significantly decreased (Pu003c0.05); lipid droplets of hair follicle appeared in the structure of hypothalamus. The number of pituitary eosinophils increased significantly, the number of basophils remained unchanged, and the area of seminiferous tubules in testicular tissue was relatively high, but the difference was not significant.

　　Conclusion: Six weeks of free exercise can effectively improve the abnormal secretion of sex hormones and the imbalance of estrogen and male hormone ratio caused by high-fat diet, but it has the effect of improving hormonal changes in the pathology of the pituitary gland-the pituitary-testicular axis is not clear.