动物造模 z-bio 2021-07-15 282

　　Objective: To investigate the inhibitory effect of highly selective M4 receptor blocker MT3 on guinea pig morphological deprivation myopia and its underlying mechanism.

　　Method: 3-week-old guinea pigs were randomly divided into 3 groups: control group, defoaming group, and defoaming+MT3 group. Before and after the experiment, a strip photometer was used to measure the optical power, A-ultrasound was used to measure the biological parameters of the eye, and RT-PCR was used to measure the relative expression of TGF-β2 mRNA in the retina and choroid.

　　Results: The guinea pigs in the deformed right eye+MT3 group developed (-1.44±0.50)D relative myopia (right eye-left eye), and the vitreous cavity and axis were lengthened by (0.10)±0.02) mm and (0.14± 0.07) mm (P=0.001, Pu003c0.001, Pu003c0.001), but the amount of myopia, vitreous cavity and axial length were significantly smaller than the increased simple deprivation group (Pu003c0.001). 001, Pu003c0.001, Pu003c0.001). Simple morphological deprivation may reduce the relative expression of TGF-β2 mRNA in the retina and choroid (P\u003c0.001, P = 0.014). On the other hand, intravitreal injection of MT3 may lead to morphological deprivation of the relative expression of TGF-β2 mRNA in the retina and up-regulate the ocular choroid (Pu003c0.001, Pu003c0.001).

　　Conclusion: MT3 can inhibit the formation of deprivation myopia in guinea pigs. It may act by up-regulating the level of TGF-β2 mRNA in the retina and choroid.