动物造模,实验动物,口腔菌群 z-bio 2021-10-12 596

　　Objective: To apply 16SRNA high-throughput sequencing method to determine the oral flora of several commonly used experimental animals (Tibetan miniature pigs, beagle dogs, macaques, New Zealand rabbits, Wistar rats), and to compare and analyze them with humans. The study of oral microbiology model provides basic information.

　　Methods: Use disposable cotton swabs to collect specimens of Tibetan miniature pigs, beagle dogs, macaques, New Zealand rabbits, Wistar rats, and human oral flora, extract the total DNA of the samples, label the universal primers, and amplify the 16SRNAV4 area for use. The fragments were sequenced by Illumina and analyzed by BIPES and QIIME to compare the diversity and structure of the flora.

　　Results: There was a significant difference in the abundance of oral flora between humans and five commonly used experimental animals (Pu003c0.05). Although each animal has its own oral flora, the oral flora of monkeys is most similar to that of humans. ..

　　Conclusion: Among the five species of animals, the levels of Fusobacterium and Porphyromonas in the oral cavity of monkeys are the most similar to those of humans. Based on their similarity to certain types of human oral flora, monkeys may be researchers. Model animals with better oral flora. In terms of specific flora, Tibetan miniature pigs may be more suitable model animals for studying Proteobacteria related diseases, while Beagle dogs are more suitable for studying Spirochetes related diseases, which can be animals.