炎性痛 z-bio 2021-07-15 250

　　OBJECTIVE: To observe the emotional response of CFA-induced inflammatory pain model rats, and to explore the relationship between the anterior cingulate cortex (ACC) phosphokinase zeta (PKCzeta) and the emotional response of inflammatory pain rats.

　　Method: 24 clean male SD rats were randomly divided into a blank control group and a model control group. The chronic inflammatory pain model was established by subcutaneous injection of Freund's complete adjuvant. The changes in body weight and pain threshold of rats before (basic) and 3, 7, 14, 21, and 28 days after modeling were dynamically observed; all were in the hyperoxic maze on 14 and 21 days. , 28 days after modeling, observe the percentage of total movement distance, open arm movement distance, open arm entry time and open arm stay time. Observe the total distance of all rats walking in the open space, the walking distance in the central quadrant, the number of entrances in the central quadrant, and the time in the central quadrant on the 14th and 29th days after modeling. At 14 and 29 days after modeling, Western blotting was used to detect the expression of PKC zeta protein in the non-participating and affected ACC regions.

　　Result: There was no significant difference in body weight between the two groups of rats at each time point (P→0.05). Before modeling, there was no significant difference in pain threshold between the two groups of rats (P\u003c0.05). One day after modeling, the pain threshold of rats in the model control group was significantly reduced (Pu003c0.05), and it was significantly reduced throughout the experiment. Blank control group (Pu003c0.05). Compared with the blank control group, the ratio of arm opening distance to arm opening time (Pu003c0.05), central quadrant movement distance and the number of central quadrants in the model control group decreased significantly after 28 days of modeling. The entry time was significantly reduced 29 days after the model (Pu003c0.05). 29 days after modeling, the expression of PKC zeta protein in the affected ACC area of the model control group was significantly higher than that of the blank control group (Pu003c0.05). And open arm travel distance, open arm residence time percentage, central quadrant travel distance, central quadrant invasion time were negatively correlated with changes in PKCzeta protein expression on the affected side. .

　　Conclusion: CFA-induced chronic inflammatory pain rats may show abnormal emotional behaviors. The emotional behavior of chronic inflammatory pain may be related to the high expression of PKCzeta in the ACC area.