动物造模,糖尿病 z-bio 2021-07-15 481

　　Objective: To study the changes in SOD activity and protein expression levels in the main organs of diabetic rats, and to observe the effect of antioxidant N-acetylcysteine (NAC) on the body's antioxidant status after short-term treatment (4 weeks). .

　　Method: STZ-induced diabetic rats (group D, n=8) received NAC 1.5 g/kg/day (group D+, n=8) and normal control group (group C, n=8). .. Give the same amount of saline at the same time. After 4 weeks, collect heart, lung, liver, and kidney tissues, and collect plasma total SOD, total antioxidant level, lipid peroxidation specific marker 15-F2t-isoprostaglandin and total superoxide dismutase ( Superoxide dismutase (SOD) was detected.) Activity, Western blot analysis of SOD subtype Cu/Zn-SOD and Mn-SOD protein expression levels.

　　Results: The plasma 15-F2t-isoprostaglandin, total antioxidant concentration and total SOD activity of myocardial tissue of group D rats were significantly higher than those of group C, but the total SOD activity of plasma, lung, liver, and lung tissues were significantly increased. Decrease; the expression level of Cu/Zn-SOD protein in heart and lung tissues increased significantly, but the expression level of liver and kidney tissues decreased significantly; the expression level of Mn-SOD protein in lung and kidney tissues decreased significantly. The expression of liver tissue was significantly increased and decreased, but the changes in myocardial tissue were unclear. AC intervention can reverse the above changes to varying degrees, but further reduce the expression of Mn-SOD in kidney tissue.

　　Conclusion: There are tissue differences in total SOD activity and Cu/Zn-SOD and Mn-SOD protein expression levels in different tissues of diabetic rats. The antioxidant NAC can restore the antioxidant levels in different tissues of diabetic rats to varying degrees. It is used to prevent or delay damage to diabetes-related target tissues.