动物造模 z-bio 2021-12-04 400

　　Purpose: This article describes the effect of the epithelial sodium channel (ENaC) on the function and activity of osteoclasts.

　　Method: Using rat macrophage colony stimulating factor and nuclear transcription factor-κB receptor activator ligand to differentiate rat bone marrow monospheres into osteoclasts. Inoculate a 12-well plate at a density of 1.5 x 104. Check the random table and divide the 3 wells into 1 group and 4 groups. The amiloride (Ami, ENaC inhibitor) group at different concentrations from the control group. Tartrate-resistant acid phosphatase (TRAP) staining was used to identify osteoclasts positive, and osteoclasts and bone fragments were co-cultured to determine the number of bone resorption cracks. RT-PCR was used to analyze the osteoclast marker enzyme gene cathepsin K (CK). formula.

　　Result: After treating osteoclasts with different concentrations of Ami, TRAP staining-positive osteoclasts decreased, inhibited osteoclast formation and bone resorption, and reduced the expression of osteoclast-specific gene CK. I was allowed.

　　Conclusion: This experiment proves that ENaC is expressed at the cellular level in osteoclasts and regulates osteoclast differentiation and bone resorption. ENaC may be involved in the regulation of osteoclast function, indicating that there is gender. The new methods of related regulation provide new ideas for bone metabolism research.