动物造模,创伤性颅脑损,神经干细胞 z-bio 2021-07-15 299

　　Objective: To investigate the time course of neural stem cell proliferation and differentiation in the subventricular zone after brain trauma.

　　Method: The rats were divided into 3 groups using a random number table method. The control group did not receive any treatment. In the sham operation group, only the skin and skull were opened. The experimental group used Finney's method to cause head injury (TBI). Using two cell markers nestin and BrdU, as well as neuron-specific markers neuron-specific enolase (NSE) and glial cell markers GFAP, three sets of brain tissue specimens were selected, using immunofluorescence, double-labeled protein Antibody runs. Immunofluorescence staining is used to detect the proliferation and differentiation of SVZ endogenous SCs after TBI.

　　Results: After TBI, SVZNestin/NSE, Nestin/GFAP, BrdU/NSE, BrdU/GFAP labeled positive cells increased significantly on the injured side, and began to increase on the first day after injury, and reached a peak on the third day. achieved. The comparison between the four time points of the experimental group and the corresponding time points of the experimental group and the control group is very important. Among them, nestin/GFAP-labeled proliferation and division positive cells are the most important increase.

　　Conclusion: After TBI, injured SVZ neural stem cells are mobilized to induce the proliferation and differentiation of endogenous neural stem cells in this area. This indicates that SVZ is one of the important germinal centers for SC proliferation and differentiation.