动物造模,蛋白磷酸酶5 z-bio 2021-09-28 909

　　OBJECTIVE: To use protein phosphatase 5 (PP5) knockout mice to study the role of PP5 in fat metabolism in mice.

　　Methods: Six-week-old male PP5 knockout mice (PP5KO) and wild-type (WT) mice were randomly selected. After 6 weeks of high-fat diet, the liver was stained with HE and Oil Red O. The lipid droplets of the mice were accumulated as follows: Detect and use Western blotting and real-time PCR technology to detect the expression of lipid metabolism-related genes in liver tissue. At the same time, PP5KO and WT mouse fibroblasts were used to observe the effect of PP5 on adipocyte differentiation in vitro.

　　Result: Compared with WT mice, the weight of PP5KO mice after a high-fat diet was significantly lower than that of WT mice, the number of liver lipid droplets was significantly reduced, and the lipid droplets were smaller. In vitro experiments showed that the fat differentiation of PP5KOMEF cells was significantly weaker than that of WT mouse embryonic fibroblasts (MEF), and the lipid droplets were smaller. In addition, in PP5KO liver tissue, the relative expression of adipogenic differentiation marker genes CD36, AP2, PPARγ2 and Glut4 were significantly reduced, and the phosphorylation level of the energy metabolism-related protein glucocorticoid receptor (GR) was significantly reduced. Uncoupling from protein 1. (UCP1) expression also increased significantly.

　　Conclusion: PP5 regulates fat metabolism in mice by regulating GR dephosphorylation and affecting body fat differentiation and energy metabolism.