动物造模,急性肺水肿大鼠 z-bio 2021-07-16 813

　　Objective: To investigate the changes of malondialdehyde (MDA), superoxide dismutase (SOD) and interleukin 6 (IL-6) in the lung tissue of a rat model of acute pulmonary edema caused by acute hypoxia.

　　Methods: 50 adult Wistar rats were randomly divided into group A (normal group), group B (acute pulmonary edema model, hypoxia for 24 hours), group C (acute pulmonary edema model, hypoxia for 48 hours), and group D entered ( Acute pulmonary edema model). , Hypoxia for 72 hours) and E group (acute pulmonary edema hormone treatment group, hypoxia for 72 hours). Rats in groups B, C, D and E were injected intraperitoneally with 6? ammonium to establish an acute pulmonary edema model. Group E received 6.0 mg/kg dexamethasone via the tail vein 30 minutes after the injection of ammonium hexamethylene. Four groups of rats were sacrificed 24 hours, 48 hours, and 72 hours after the successful modeling. The rat plasma was separated and the rat lung was taken to prepare a 10? tissue homogenate to detect MDA, SOD, and IL-6 in the lung tissue. By ELISA and the IL-6 content and activity in plasma.

　　Results: The three groups B, C and D showed obvious edema and wet weight increase in the lung tissue of rats. Compared with group A, the difference was statistically significant (P0.05). , Rats in the MDA D group increased lung tissue, SOD decreased, and plasma IL-6 increased. The change was significant, and the difference was statistically significant compared with group A (Pu003c0.05). Compared with group D, lung tissue MDA of rats in group E decreased, SOD increased, and plasma IL-6 decreased, the difference was statistically significant (Pu003c0.05).

　　Conclusion: The occurrence of acute pulmonary edema is related to oxidative stress. The decline of the body's antioxidant capacity and the increase of free radicals are important mechanisms for the occurrence of pulmonary edema. Lung tissue has the importance of directing disease changes.