大鼠脑缺血再灌注神经损伤 z-bio 2021-07-16 511

　　Objective: To study the effect of Huang injection (AI) on the apoptosis of hippocampal neurons in rats with hypoxia/hypoglycemia/levoxyglucose (H/R) under the action of Bcl-2 inhibitor (TW-37).

　　Methods: The primary cultured 8d fetal rat hippocampal neurons were randomly divided into 6 groups: normal control group, H/R group, AI group, AI solvent control group, Bcl-2 inhibitor (TW-37) + H/ Group, TW-37+AI+H/R group. Except for the normal control group, each group experienced hypoxia and hypoxia for 0.5 hours, after which the indicators were 7 time points after reoxygenation and resuscitation (0 hours, 0.5 hours, 2 hours, 6 hours, 24 hours, 72 hours). 120 hours). The MTT method was used to detect cell viability, and Western blot and RT-PCR were used to detect the expression of caspase-3 protein and mRNA in hippocampal neurons.

　　Result: Compared with the normal control group, the hippocampal neuron activity in the H/R group was significantly reduced except for 0 hours (P0.05).

　　Conclusion: Ranger injection can enhance the activity of hippocampal neurons in H/R rats, activate the anti-apoptotic mechanism of Bcl-2, and reduce the expression of caspase-3. Bcl-2 is a ranger injection that can inhibit the hippocampal neurons of Helicobacter pylori. /R One of the important targets of rat cell apoptosis.