动物造模,兔肝动脉插管 z-bio 2021-07-16 335

　　Objective: To explore the way and method of hepatic artery cannulation in experimental rabbits.

　　Method: 30 New Zealand white rabbits were randomly divided into 5 groups: peripheral intravenous injection, hepatic artery perfusion, hepatic artery lipiodol embolization, hepatic artery gelatin sponge particle embolization, liver direct puncture injection... an effective amount of limonene injection. The hepatic artery cannula uses Seldinger's surgical procedure to puncture the ear or femoral artery, respectively, depending on the odd and even number of experimental rabbits. If one method fails, you can switch to another puncture method. Record the insertion time, success rate and complications of the puncture catheter and hepatic artery catheter. For pharmacokinetic analysis, venous blood was collected from all experimental rabbits during multiple periods after administration.

　　Results: The success rate of femoral artery puncture in rabbit ears was 55.6-5/9, 2 on one side, 3 on both sides) and 88.9-16/18, 12 on one side, 4 on both sides). The rate of intubation through the rabbit ear and the femoral artery and hepatic artery were 0? 0/5) and 100? 16/16), and the operation time was 28.4±13.6 and 33.9±19.6 minutes, respectively. Except for the direct liver puncture group and the femoral artery puncture group, one animal died accidentally due to anesthesia, and one animal was euthanized after the arterial puncture failed. The other experimental rabbits did not die. After general anesthesia, the peripheral blood vessels of the experimental rabbits contracted significantly, and it was difficult to collect blood from the rabbit ear veins in a short time. In the experiment, 23 rabbits (23/27) were modified to draw blood. Blood from the jugular vein.

　　Conclusion: It is unlikely that the hepatic artery can be intubated through the rabbit ear artery. Femoral artery intubation is the first choice for interventional physicians. Rabbit jugular vein blood sampling has unique anatomical advantages and high experimental value.