角膜移植排斥反应,基质细胞衍生因子-1 z-bio 2021-07-16 653

　　Objective: To investigate the expression of stromal cell-derived factor-1 (SDF-1) and stromal cell-derived factor-1 (CXCR4) in rat corneal tissues and their role in immune rejection after corneal transplantation.

　　Methods: 15 Wistar rats were the normal control group, 22 Wistar rats were the autologous group, 22 SD rats and 44 Wistar rats were the donors of SD rats, and the translucent cornea was the recipient... Twenty-two rats in the Dianbishu group were randomly selected. Apply Dianvis eye drops to your eyes (twice a day). The remaining 22 animals were classified into the allogeneic group. Inject the same amount of saline. One month's eyes. On the 5th and 9th days after surgery, the Larkin clinical evaluation method was used to conduct histopathological observation, immunohistochemical examination, and real-time fluorescence quantitative PCR detection on the corneal grafts in each group.

　　Result: There is no denial in my group. The corneal survival time of the Dianbishu group was 24±0.32d, which was 10±0.36d longer than that of the allogeneic group (Pu003c0.001). Histopathological examination revealed a large number of inflammatory cell infiltration and angiogenesis in the cornea of the allogeneic group. The expression levels of SDF-1 and CXCR4 mRNA in allogeneic corneal tissues were significantly increased (Pu003c0.001 on day 5 and Pu003c0.01 on day 9), but the expression levels were significant in the rhinoelectric relaxation group and allogeneic corneal tissues reduce. Immunohistochemical examination showed that SDF-1/CXCR4 was mainly expressed in the epithelial and mesenchymal layers of corneal grafts, and the content of SDF-1 and CXCR4 in allogeneic corneal tissue was significantly increased.

　　Conclusion: SDF-1/CXCR4 may be involved in the early rejection after rat corneal transplantation. The mechanism may be that SDF-1 specifically induces CXCR4+ cell maturation and chemotaxis to rejection sites, and promotes corneal neovascularization.