动物造模 z-bio 2021-07-16 792

　　OBJECTIVE: To study the distribution and differential expression of Hba-α protein in hatched blastocysts and dormant embryos of mice before and after procedural freezing, and to provide a theoretical basis for the future development and development of new mammalian antifreeze proteins.

　　Method: Obtain hatched blastocysts from pregnant d5 mice, obtain dormant embryos from delayed implantation models of mice, and use confocal microscope and Western Blot technology to detect programmed frozen expression. Perform the distribution of Hba-α protein and the distribution of each group before and after the embryo.

　　Result: Both hatched blastocysts and dormant embryos before and after the program freezing express Hba-α protein. The expression of Hba-α protein in blastocysts hatched after program freezing was not significantly different from that before freezing (P\u003c0.05); Hba-α protein expression was significantly down-regulated in dormant embryos hatched before and before and after program freezing. Blastocysts (Pu003c0.05); Hba-α protein expression of resting embryos after freezing was significantly lower than that of blastocysts hatched before and after the program freezing. The other group (Pu003c0.05).

　　Conclusion: The effect of programmed freezing on Hba-α protein expression in dormant mouse embryos is significantly greater than that of hatched blastocysts. Hba-α gene is a new type of mammalian abstinence gene.