动物造模,巨噬细胞,溃疡性结肠炎 z-bio 2021-07-16 1037

　　Objective: To explore the role of macrophage NF-κB activator 1 (nuclearfactorkappaBactivator1, Act1) in inflammatory bowel disease.

　　Method: Targeting mice that inhibit the expression of Act1 (anti-Act1) in macrophages, using dextran sodium sulfate (DSS) to induce ulcerative colitis model, mouse body weight and stool changes. Colon and rectum HE stained tissues assess the severity of inflammation, such as measuring blood, stool viscosity based on disease activity index scores, immunohistochemical methods to analyze leukocyte and macrophage infiltration, and T-qPCR to detect changes in the expression of related cytokines.

　　Results: Compared with C57 mice 7 days after DSS induction, anti-Act1 mice had lower colorectal inflammation index scores, fewer macrophages and other infiltrations, and higher expression of TNF-α, IL-1β, and IL-6 mRNA. low.

　　Conclusion: Targeted inhibition of Act1 expression in macrophages can reduce DSS-induced ulcerative colitis.