动物造模,神经干细胞 z-bio 2021-07-19 629

　　Objective: To clarify the neurobiological function of LSD1 by conditionally knocking out the LSD1 gene in mouse neural stem cells to observe the proliferation of mouse neural cells and mouse behavior.

　　Method: Pair LSD1 (flox/flox) transgenic mice with estin-cre (Tg) transgenic mice expressing Cre recombinase dedicated to neural stem cells. That is, it uses the Cre-LoxP system to replicate LSD1 (flox/flox) Nestin-cre (Tg) genotype mice, that is, the required neural stem cell LSD1 conditional knockout mice (LSD1-CKO), and control LSD1 (flox/flox/ flox) as a mouse. In addition, immunofluorescence is used to detect the proliferation of hippocampal DG neurons in mice, and to detect mouse behavior through experiments such as sugar water preference, forced swimming, and recognition of new objects in mice.

　　Results: Compared with LSD1 (flox/flox) mice, the proliferation of hippocampal neurons in LSD1-CKO mice was significantly reduced (P=0.023); the sugar water preference coefficient was reduced (P=0.0075), unshakable. The time of the forced swimming experiment was significantly increased (Pu003c0.05), and the new object recognition experiment showed a significant decrease in memory (P=0.0019).

　　Conclusion: Knockout of LSD1 gene in mouse brain neural stem cells reduces the proliferation of hippocampal nerve cells. LSD1-CKO mice exhibit negative emotions and memory deficits.