动物造模,人羊膜间充质干细胞移植,慢性酒精性肝损伤 z-bio 2021-07-19 577

　　Purpose: Studies have shown that human amniotic membrane mesenchymal stem cells have a variety of differentiation and proliferation functions, and induce differentiation into liver-like cells, thereby resisting liver injury.

　　Method: Human amniotic membrane mesenchymal stem cells were resuscitated and cultured in vitro. 66 healthy female Wistar rats were randomly selected as normal controls. 22 of them were untreated. The remaining 44 were treated with liquor and forced to feed chronically for 30 minutes. Alcoholic liver disease models were randomly divided into model group (tail vein injection of 1 mL PBS) and hAMSC group (tail vein injection of 1 mL hAMSC (2×106), 22 in each group). 4 weeks after transplantation, use an automatic biochemical analyzer to detect serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum total bilirubin (TBIL), serum albumin (ALB), and serum total protein (Tp). . Changes in the contents of malondialdehyde and IL-4 in superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and rat group (MDA); PKH-26 immunofluorescence microscopy was used to observe its distribution, hAMSC, TUNEL method to detect hepatocyte apoptosis

　　Result: The blood biochemical test results of rats in each group were all normal. The contents of ALT, AST, TBIL, and Tp in the model group were significantly higher than those in the control group, but the contents of ALT, AST, TBIL, and Tp in the hAMSCs transplantation group were higher than those in the model group. Compared with the normal control, it was significantly lower (Pu003c0.05). The ALB content of the model group was significantly lower than that of the model group, but the ALB content of the hAMSCs transplantation group was significantly increased (Pu003c0.05). 05); Compared with the model group, the contents of SOD, GSH-PX and CAT in the hAMSCs transplantation group were significantly higher than those in the hAMSCs transplantation group. The contents of SOD, GSH-PX and CAT were all decreased, and MDA and IL-4 were all increased (Pu003c0.05) ). The PKH-26-labeled positive hAMSC cells in the transplantation group were distributed in the liver tissue after transplantation, but this cell distribution was not seen in the other groups (Pu003c0.05). The TUNEL method detects the number of apoptotic cells in each hepatocyte apoptosis. The model group was the most common (34.27±5.71), followed by the hAMSCs transplantation group (18.42±3.95). No apoptotic cells were found in the normal control group.

　　Conclusion: Transplantation of human amniotic membrane mesenchymal stem cells improves the blood biochemical indicators of liver and liver diseases in rats. hAMSC transplantation can reduce hepatocyte apoptosis in rats with chronic alcoholic liver disease.