动物造模,兔急性肾损伤 z-bio 2021-07-19 396

　　Objective: To investigate the course of acute kidney injury (AKI) in rabbits caused by a single intravenous injection of cisplatin.

　　Method: 40 New Zealand male rabbits were randomly divided into 5 groups, each with 8 rabbits. A single injection of cisplatin (5 mg/kg) from the ear vein was used to create an acute kidney injury model. According to the observation time, they are divided into 6-hour group (T6), 12-hour group (T12), and 24-hour group. After the cisplatin injection group (T24), the 36-hour group (T36) and the 48-hour group (T48), the rabbit intravenous urea nitrogen (BUN) and blood creatinine (SCr) levels were measured at the corresponding time points before and after the injection. It was completed. ,respectively. We use SPSS 22.0 statistical software for statistical analysis of the data. Pu003c0.05 indicates that the difference is statistically significant. At each experimental node, at the expense of experimental rabbits, kidney tissue specimens of each group of rabbits were taken, and normal paraffin sections were observed under HE staining light microscope.

　　Results: After cisplatin injection, the levels of BUN and SCr in each group were significantly higher than those before injection. 24 hours after modeling, the experimental rabbits in each group reached the acute kidney injury standard, and the injury gradually worsened. When observed with an HE stained optical microscope, the changes in the T48 group were more obvious.

　　Conclusion: A single intravenous injection of cisplatin can cause acute kidney injury in rabbits, which gradually worsens within 48 hours.