动物造模,实验性自身免疫性脑脊髓炎 z-bio 2021-07-19 425

　　OBJECTIVE: To observe the effects of different concentrations of baicalin on experimental autoimmune encephalomyelitis (EAE) mouse models, and to preliminarily explore its mechanism of action.

　　Method: Establish a mouse model of experimental autoimmune myelitis. On the third day after immunization, high and low doses of bacitracin were given once a day for 20 days. Mouse activity nerve function score, TUNEL staining to detect spinal cord tissue apoptosis, ATP level detection kit to detect spinal cord tissue ATP content, Bax, Bcl-2, cleavedcas-9 and cleavedcas-3 to detect Western blotting protein expression levels.

　　Results: Bicin can improve the neurological function of experimental autoimmune encephalomyelitis mice and delay its onset time; after Vitin intervention, tunnel staining results show that the number of apoptotic cells in spinal cord tissues Western blotting results show that the level of ATP decreased and Bcl-2 protein The expression increased significantly, and the protein expression of Bax, cleavedcas-9 and cleavedcas-3 decreased significantly. I proved it.

　　Conclusion: Visine inhibits endogenous mitochondrial apoptosis, protects mitochondria, improves the neurological function of experimental autoimmune encephalomyelitis mice, thereby inhibiting cell apoptosis. This provides an experimental theoretical basis for disease prevention.