动物造模,Fkbp51基因敲除 z-bio 2021-09-23 549

　　Objective: To study the effect of Fkbp51 knockout (KO) and wild-type (WT) mouse liver expression profiles on alternative splicing of liver tissue genes.

　　Method: Use next-generation sequencing to sequence the liver expression profiles of Fkbp51KO and WT mice, and use TopHat to perform alternative splicing analysis on the RNA sequencing results of KO and WT mice to screen for differences in liver tissue intron retention. ,I) and exon skipping (SE). Use the online tool DAVID to perform gene ontology (GO) and metabolic pathway (kyotoencyclopediaofgenesandgenomes, KEGG) enrichment analysis for these different alternative spliceosomes, and use the CBI gene database to annotate these genes.

　　Results: (1) Fkbp51 deletion can cause changes in mouse liver mRNA alternative splicing; (2) Fkbp51 gene knockout can cause changes in mouse liver mRNA alternative splicing expression; 3) through GO and KEGG analysis, alternative splicing of these genes It is mainly related to the pathways of metabolism, immunity, bile acid secretion and other fat-related derivatives. (4) Genes related to different intron retention are mainly related to the regulation of actin cytoskeleton and the metabolism of amino acids and their derivatives.

　　Conclusion: Fkbp51 gene knockout will change the alternative splicing of mRNA in the genome, thereby affecting the metabolic function of the mouse liver.