动物造模 z-bio 2022-01-17 790

　　Objective: To investigate the effect of intrauterine injection of absolute ethanol on the estrous cycle of mice.

　　Method: Select 20 mice, use vaginal desquamation cell staining for regular estrus and intrauterine ethanol injection. The estrus cycle was measured every day during the first estrus cycle after the operation, and 10 mice were randomly selected during the second estrus cycle. Measure the cycle every two hours to observe the degree of change in the estrus cycle.

　　Result: After the experimental mice were injected with absolute ethanol in utero, the overall estrus cycle was significantly prolonged, and various degrees of estrus, pre-estrus and other stages of disorder appeared.

　　Conclusion: This experiment provides specific experimental evidence for the clinical application of absolute ethanol by observing that the uterus cycle of mice is damaged after uterine injury caused by absolute ethanol.