动物造模 z-bio 2021-07-20 392

　　OBJECTIVE: To investigate the inhibitory effect of deoxyionization on the proliferation and inducing apoptosis of human colon cancer HT29 cells and its possible mechanism.

　　Method: Treat HT29 cells with different concentrations of deoxygenated violin, and use the tetramethylazolyl blue (MTT) colorimetric method to detect the inhibition rate of HT29 cells on deoxyviral protein. Flow cytometry is used to detect cell cycle and apoptosis. Use Western blotting to detect changes. Expression of apoptosis-related proteins Bax, Bcl-2 and Caspase-9.

　　Result: Deoxypurpurin has a significant inhibitory effect on the proliferation of HT29 cells. This increases the ratio of G0/G1 cells and decreases the ratio of S phase cells. The apoptotic rate increased, and the expression of Bax and Caspase-9 increased. Protein increases and Bcl-2 protein expression decreases.

　　Conclusion: Deoxygen violin inhibits the growth of colon cancer HT29 cells and induces apoptosis, and its apoptosis mechanism may act on the endogenous pathway mediated by mitochondria.