动物造模,大鼠脑缺血再灌注损伤 z-bio 2021-07-20 480

　　Objective: To investigate the effect of hydrogen-rich liquid on hippocampal neuron apoptosis and PI3K/Akt/FoxO1 signal pathway expression induced by cerebral ischemia-reperfusion in rats.

　　Method: The middle cerebral artery of the rat was sutured and occluded to establish a local cerebral ischemia reperfusion (I/R) model. SD rats were randomly selected in the sham group and the ischemic group. Be divided into. / Reperfusion group (I/R group) and hydrogen-rich solution treatment group (HRS group), each with 10 animals; 24 hours after reperfusion in each group, serum inflammatory factors IL-6, TNF-α and IL-Is were detected. ELISA1β changes; HE staining to observe hippocampal changes, TUNEL method to observe brain cell apoptosis; Western detection of hippocampal p-PI3K, Akt, caspase-3 and FoxO1 protein expression changes.

　　Results: Compared with the sham group, the pyramidal cells in the I/R group were loosely arranged, a large number of pyramidal cells died, hippocampal apoptotic cells increased, the expression of serum inflammatory factors IL-1β and IL-6 increased, and TNF-α increased significantly . (Pu003c0.05), the expression of p-PI3K, Akt, and caspase-3 in brain tissue increased significantly, and FoxO1 protein decreased. There are statistical differences between the two groups. (Pu003c0.05); HRS group inflammatory factors were significantly lower than I/R group; p-PI3K, Akt, caspase-3 expression levels were significantly reduced, FoxO1 protein increased (Pu003c0.05). 05).

　　Conclusion: Hydrogen-rich liquid can reduce brain damage caused by ischemia-reperfusion. This mechanism may be related to the PI3K/Akt/FoxO1 signaling pathway.