动物造模,大鼠胸主动脉血管平滑肌细胞 z-bio 2021-07-20 716

　　OBJECTIVE: This article reports an improved method for isolating rat thoracic aortic vascular smooth muscle cells based on complex enzyme digestion.

　　Method: Digest the thoracic aorta with collagenase and tryptic peptidase complex enzyme, remove the intima and adventitia, and then digest the medium again to release vascular smooth muscle cells.

　　Result: After the cells are separated, they grow adherently, showing a spindle shape, showing typical "peak-to-valley" growth, and can be subcultured within 1 week. A variety of molecular marker genes for contracting vascular smooth muscle cells have been isolated, and they are highly expressed in cells. Cells above 95° are significantly positive for α-SMA and myosin-II.

　　Conclusion: This separation method is simple, easy to implement, reproducible, and the obtained cells have high viability and high purity, so a large number of contractile vascular smooth muscle cells can be obtained in a short time.