动物造模 z-bio 2021-09-26 848

　　Objective: To investigate the expression level and importance of maspin and IKKα in the mucosal tissues of the sinuses of rats with fungal rhinosinusitis (FRS).

　　Method: 40 SD rats were established as fungal sinusitis models, and they were divided into nasal congestion group, FRS group, immunosuppressant group, and aggressive FRS group according to the random number table method, with 10 rats in each group. Serve. There are 10 rats in each group. Healthy rats served as a blank control group. The control group was fed normal food. In the nasal congestion group, hemostatic cotton was inserted into the nasal cavity, and the same amount of 0.9?Cl was injected intraperitoneally. Nasal cavity; the FRS group was injected with the same amount of 0.9?Cl injection into the intraperitoneal cavity, and the nasal cavity was injected with the Aspergillus tobacco spore suspension; the immunosuppressant group was injected with the same amount of 0.9?Cl and cyclophosphamide intraperitoneally; cyclophosphamide was injected into the peritoneal cavity of the aggressive FRS group and the nasal cavity The invasive FRS rat model was established by injection of Aspergillus fumigatus spore suspension. Enzyme immunoassay was used to detect the expression levels of serum interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α). Immunohistochemical staining was used to detect the expression levels of maspin and IKKα proteins in the nasal cavity. For each group of rats, fluorescence real-time PCR was used to detect the expression levels of Maspin mRNA and IKKα mRNA in the nasal cavity of each group of rats.

　　Result: The expression levels of serum IL-6 and TNF-α of rats in each group are significantly different (P\u003c0.05). Immunohistochemical staining results showed that the expression level of maspin protein in the FRS group and the invasive FRS group was significantly lower than that of the control group, nasal congestion group and the immunosuppressive group, and the expression level of IKKα protein was significantly higher than that of the control group. The maspin protein expression in the invasive FRS group was significantly lower In the FRS group, the expression of IKKα protein was significantly higher than that in the control group, nasal congestion group, and immunosuppressive group (Pu003c0.05). The FRS group (Pu003c0.05). The expression level of maspin mRNA in FRS group and aggressive FRS group was significantly lower than that of control group, nasal congestion group and immunosuppressive group. The expression level of IKKα mRNA was significantly higher than that of the control group and the nasal cavity. The expression of maspin mRNA in the hyperemia group and the immunosuppressive group (Pu003c0.05), the aggressive FRS group was significantly lower than the FRS group, and the expression of IKKα mRNA was significantly higher than the FRS group. FRS group. (Pu003c0.05).

　　Conclusion: The down-regulation of maspin expression after activation of IKKα is an important cause of the pathogenesis of FRS, and this process may also be one of the molecular mechanisms of FRS invasiveness.