动物造模 z-bio 2021-07-20 406

　　OBJECTIVE: By screening the missing chromosome 12 markers in the local standard DB11/T828.3-2011, comparing the genetic evaluation of different markers for the same population of miniature pigs in Beijing area, and analyzing the applicability of landmarks.

　　Method: Combined with literature reports, select 4 pairs of microsatellite markers on chromosome 12 to screen 2 Chinese agricultural and piglet populations. Select the highly polymorphic chromosome 12 markers, and use standard methods to combine 18 pairs of chromosome markers into a whole. The chromosomal method is used to compare and analyze the genetic quality assessment of populations in the same batch in different ways. The results obtained are processed and analyzed using Popgen32 software.

　　Result: The PIC values of the selected 4 pairs of chromosome 12 markers are all above 0.5, which is a high degree of polymorphism. The current standards for chromosome coverage, amplification stability and result evaluation are 94.7 96.0 respectively. Nongda I line is unqualified and Nongda III line is qualified. After adding chromosome 12, the evaluation results of the three indicators were 100-96.6. Both groups were qualified.

　　Conclusion: The 4 pairs of chromosome 12 markers selected in this study are highly polymorphic, and provide data support for the improvement of DB11/T828.3-2011.