动物造模,乳腺癌肿瘤血管正常化 z-bio 2022-01-31 636

　　Objective: To study the effect of a single intraosseous injection of simvastatin on the vascular structure and function of breast cancer tumors in mice.

　　Method: In order to establish an orthotopic model of mouse breast cancer, simvastatin (50 μg) or a blank carrier was injected into the bone at a time. α-SMA/CD31 immunohistochemical double staining was used to observe tumor vascular peripheral/vascular endothelial cell coating; Evans blue dye was injected into the tail vein to observe tumor vascular permeability; HIF-1α immunohistochemistry was used to detect changes in tumor expression levels.

　　Results: Compared with the control group, immunofluorescence double staining increased the coverage of tumor endothelium in the simvastatin group (Pu003c0.05), and significantly reduced the Evans blue content in the tumor tissue (Pu003c0..05), indicating tumor blood vessels The permeability decreases and the tumor blood vessels tend to mature. Immunohistochemistry has been shown to reduce the expression level of HIF-1α in tumors and improve hypoxia in breast cancer tissues.

　　Conclusion: A single intraosseous injection of simvastatin may increase the pericyte coverage of new blood vessels and promote the normalization of breast cancer tumor blood vessels.