动物造模,黑色素瘤 z-bio 2021-09-26 719

　　Objective: To study the effect of Mac-1 deletion on the growth of melanoma.

　　Method: Extend your Mac-1 Knockout (Mac-1-/-) mouse to get as many mice as you need for the experiment. Establish a control group of C57BL/6J mice and an experimental group of Mac-1-/- mice, subcutaneously inject B16-F10 cells, calculate the survival rate of mice, measure tumor volume and weight, and determine tumor cells, and immunohistochemical staining to detect proliferation And tumor cell proliferation. Macrophage infiltration.

　　Result: The survival rate of melanoma mice after removal of Mac-1 is significantly different from that of control C57BL/6J mice (Pu003c0.001), and the tumor volume and weight are significantly different from that of control C57BL. In 6J mice (Pu003c0.001), the number of proliferating cells in the tumor tissue was significantly different from that in the control group (Pu003c0.01), and the infiltration of macrophages into the tumor tissue was significantly reduced.

　　Conclusion: The deletion of Mac-1 gene significantly inhibits the growth of melanoma.