动物造模,小鼠骨关节炎 z-bio 2021-07-21 567

　　Objective: To establish a related mouse osteoarthritis model through SIRT1 gene knockout, and to observe the difference in cartilage tissue slice morphology through various single or combined staining techniques on the basis of this model.

　　Methods: The mouse knee joint tissue samples were divided into two groups: SIRT1-/- mouse sham operation group (n=6, group A), SIRT1-/- mouse osteoarthritis model group (n=6, group B) ). Anterior cruciate ligament amputation and medial meniscus resection were used to establish knee osteoarthritis model. HE staining, Safranine O-fast green staining, Safranine O-Alcian blue staining, Safranine O staining, Fast green staining, AlliNeo blue staining 6 kinds of single or combined staining methods to observe the morphological changes of the cartilage tissue of the knee joint..

　　Results: Using Safranin O-fast green staining and Safranin O-alcian blue staining, chondrocyte morphology, cartilage layered structure, type II collagen fiber display, tidal line and subchondral bone changes, the effect is better; Safranin O Staining and Alcian blue staining have certain advantages in observing the loss of cartilage tissue.

　　Conclusion: When observing the knee osteoarthritis cartilage tissue sections of SIRT1 knockout mice, composite staining has a more obvious advantage in obtaining various cartilage structure information than single staining.