动物造模,小胶质细胞炎症 z-bio 2021-07-21 280

　　OBJECTIVE: To observe the effect of sodium metavanadate (NaVO3-2H2O) exposure on the inflammatory response and migration of microglia, and to explore the related mechanisms of vanadium neurotoxicity.

　　Method: Primary cultured SD rat microglia were incubated with sodium metavanadate. Immunofluorescence showed the morphological changes of microglia and the expression of specific marker Iba1. Western blotting was used to detect iNOS and COX-2. ERK and p-ERK protein expression, enzyme-binding immunosorbent assay to detect the release levels of inflammatory factors TNF-α and IL-1β, construct a scratch migration model, and record the effect of mevanazine sodium on the migration of microglia by immunofluorescence technology.

　　Results: After incubating microglia with sodium metavanadate, the morphology of microglia changed from a dormant state to a phagocytic cell, and the expression of its specific marker Iba1 was significantly increased; iNOS and proteins TNF-α and IL- The expression of 1β was significantly increased compared with the COX-2 control group; sodium metavanadate promoted the migration of microglia.

　　Conclusion: Sodium metavanadate significantly promotes the inflammatory response and migration of microglia.