动物造模,免疫抑制 z-bio 2021-07-21 389

　　Objective: To study the changes of metabolic pathways during the immunosuppressive effect of cyclophosphamide on mice by metabolomics methods.

　　Method: Use flow cytometry to understand the changes of immune cells and establish a multi-results partial least squares model for decision-making. Screen the potential biomarkers of different importance in the projection (VIP) parameters of the partial least squares model, determine the difference of metabolites through statistical analysis, and quickly identify and analyze the software system of small molecule compounds with the help of metabolomics. Finally, nine different metabolites were determined through database search and calculation, and three different metabolic pathways were analyzed from the pathway enrichment results.

　　Result: Cyclophosphamide is a normal body of metabolic pathways such as unsaturated fatty acid biosynthesis, mitochondrial fatty acid metabolism, glycerophospholipid metabolism, steroid hormone biosynthesis, arachidonic acid metabolism, fatty acid metabolism, pyrimidine biosynthesis and other metabolic pathways. It has all metabolic pathways. It has obvious effects.

　　Conclusion: The most important metabolic pathways that cyclophosphamide affects in the normal group and the experimental group are arachidonic acid metabolism, glycerophospholipid metabolism and pyrimidine metabolism.