动物造模,膀胱癌大鼠 z-bio 2021-07-21 548

　　Objective: To analyze the chemical intervention and mechanism of curcumin on N-methylnitrosourea (MNU) induced bladder cancer rat model.

　　Method: 100 SD rats were randomly divided into 4 groups, the control group (10), the model group (10), the intervention group (40), and the treatment group (40). The control group had the same time. Intravesical infusion of physiological saline and the other three groups of intravesical infusion of MNU all induced the formation of SD rats' bladder cancer model (injection of 1 mg/mL MNU solution into the bladder), but the time of MNU infusion was the second, fourth, and fourth time , 6 weeks and 8 weeks, 2 mg each time, once every 2 weeks, 4 times in total). When rat bladder cancer is induced, the model group is perfused with normal saline. , When the bladder was perfused with MNU, the intervention group was perfused with curcumin solution (400 μmol/L). That is, the bladder was perfused at 1, 3, 5, 7 and 9 weeks, and the rats were euthanized on the 10th day. Zhou: After induction of bladder cancer model in the treatment group, the bladder was injected with curcumin solution (400 μmol/L). Bladder perfusion lasted 14, 16, and 18 weeks. The rats were sacrificed on the 19th. The obtained bladder tissue was stained with hematoxylin-eosin (HE) to observe the pathological changes, and the tumor tissue apoptosis was determined by the TUNEL end labeling method. Western blot was used to detect the expression of apoptosis-related proteins.

　　Results: The incidence of bladder cancer in the model group at the 10th week was 90-9/10), and the incidence of rat bladder cancer in the intervention group at the 10th week was 12.5-5/40). used to be. The incidence of bladder cancer in the treatment group at the 10th week was 92.5~37/40). There is a significant difference in the incidence of bladder cancer between the intervention group and the model group (Pu003c0.05), and curcumin has a greater impact on MUN-induced bladder cancer. Rats have obvious chemical intervention effects. After bladder cancer formed, the treatment group was given curcumin, the incidence of bladder cancer at 19 weeks was 78.4-30/37). ) Compared with the 10th week before treatment, curcumin has been shown to be effective for bladder cancer. It has a therapeutic effect and can slow the progression of bladder cancer. The TUNEL experiment confirmed that curcumin significantly promotes the apoptosis of bladder cancer cells and inhibits the growth of bladder cancer cells. Western blot results showed that curcumin inhibited NF-κB activation and effectively down-regulated the expression of NF-κB-regulated gene products.

　　Conclusion: Curcumin has a significant chemical intervention effect in MNU-induced bladder cancer rat model. The mechanism of action is to inhibit the activation of NF-κB and effectively down-regulate the expression mechanism of NF-κB regulating gene products. The proteins in cancer mediators may regulate and regulate the bladder, inhibit growth, induce cell apoptosis, and exert anti-cancer chemical intervention to prevent the recurrence of bladder cancer.