动物造模 z-bio 2021-07-22 430

　　Objective: To compare the effects of different intervention temperatures on endoplasmic reticulum stress (ERS) and apoptosis of pressure ulcer cells in rats, and to provide experimental evidence for clinical prevention and treatment of pressure ulcers?

　　Methods: 40 SPF adult male SD rats of pressure ulcer model were randomly divided into 4 groups by ischemia-reperfusion method: sham operation group (sham operation group), model group, heat intervention group, cold intervention group. At the end of the experiment, each group was large The rat cuts off on the ice. Use HE staining to observe the pathological changes of skeletal muscle. Using Western blot to analyze the endoplasmic reticulum stress-related protein GRP78? Detect the expression level of caspase-12 and CHOP. Use immunofluorescence to observe the expression of caspase. -12 and CHOP; observe the TUNEL staining status of apoptosis?

　　Result: Compared with the model group, the skeletal muscle cell damage in the heat intervention group increased, and the number of apoptotic cells increased. The immunofluorescence result was positive. casPase? The expression of 12 and CHOP increased. Western blot results showed that the expression of GRP78? CHOP and CHOP was higher than that of the model group (Pu003c0.05), and showed the degree of skeletal muscle cell damage during cold intervention. The immunofluorescence result showed that the positive expression of caspase-12 and CHOP was decreased, and the Western blot result showed GRP78? We found that the expression of caspase-12 and CHOP were both low. Model group (Pu003c0.05)?

　　Conclusion: Local cold intervention reduces pressure ulcer injury in rats by blocking the apoptotic pathway mediated by endoplasmic reticulum stress. Local heat intervention promotes the development of endoplasmic reticulum stress and exacerbates pressure ulcer injury in rats.