动物造模,缺氧缺血性脑病大鼠模型 z-bio 2021-07-22 744

　　OBJECTIVE: To establish a hypoxic-ischemic encephalopathy (HIE) model in neonatal rats to show the brain protection of recombinant human erythropoietin (EPO) and the changes in the diversity of HIE intestinal flora in neonatal rats. The clinical application of EPO in the treatment of neonatal hypoxic-ischemic encephalopathy?

　　Method: Select 7-day-old SD rats to make HIE model, and group them into HIE model group? EPO experimental group? Randomly divided into control group, immunohistochemistry. How to observe the changes in nestin expression, collect the feces of rats in each group at the same time, and observe the changes in intestinal flora through 16sRNA sequencing?

　　Results: The Nestin expression levels of rats in each group are significantly different at the same time point (Pu003c0.05), the control group is the lowest, the EPO experimental group is the highest, and the HIE model group is the next? The Shannon Wiener index of the HIE model group is lower than that of the control group?

　　Conclusion: Exogenous administration of EPO promotes the growth of neurons in the HIE neonatal rat model and provides certain protection. At the same time, has the diversity of the intestinal flora of rats changed?