动物造模,大鼠门静脉高压 z-bio 2021-09-27 943

　　Objective: To study the effect of simple portal hypertension on rat serum endotoxin and intestinal mucosa?

　　Method: 16 rats were selected and divided into blank control group (4 rats) and model group (3d group? 7d group? 10d group, 4 rats). Each group), the model group constructed part of the portal vein ligation model, 3d-7d-10d blood collection and rat jejunum, intestine, colon, endotoxin ELISA to detect the serum endotoxin content, and observe the changes. Intestinal tissue and histopathological observation and analysis of different parts of each group for HE staining?

　　Result: Did the rats in the model group successfully construct a partial portal vein ligation model? We compared the serum endotoxin results at three different time points. Is there an obvious difference between the two intestines? There was no obvious damage to the intestinal part after 3 days of molding; all parts of the intestine had local damage and local swelling after 7 days of molding, and the villi of the intestinal lamina propria on the third day. There is a loose condition. Is the injury of the intestinal mucosa and lamina propria of the model 10d more obvious than that of the 3d and 7d?

　　Conclusion: In the case of normal liver function, portal hypertension will cause intestinal mucosa in a short time, but the endotoxin produced by the intestine does not exceed the processing capacity of the liver, increasing serum endotoxin.