动物造模,全脑缺血再灌注损伤 z-bio 2021-07-22 646

　　Objective: To study the protective effect and mechanism of cysteinyl leukotriene receptor antagonist (Plukast, HAMI3379) on global cerebral ischemia-reperfusion injury in gerbils.

　　method: bilateral common carotid artery ligation for 10 minutes, reperfusion for 24 hours, the establishment of snake global cerebral ischemia reperfusion injury model, sham operation group, model group, pull casting, and randomly divided into HAMI3379 group. Twenty rats in each group received intraperitoneal injections 3 days before surgery, once a day, and 30 minutes before surgery. After 24 hours of reperfusion, neurological symptoms and function were observed; Nistle staining to observe cortex and hippocampal neurons; Western blotting to detect the expression of cortex and hippocampal autophagy-related proteins beclin-1 and LC3; electron microscopy to observe hippocampal autophagosomes. Results Compared with the model group, the Plugast and HAMI3379 groups improved the scores of neurological symptoms, reduced nerve damage, reduced the damage and loss of cortex and hippocampal neurons, and the number of autophagy-related proteins beclin-1, LC3 and hippocampal autophagosomes cut back.

　　Conclusion: Cysteinyl leukotriene receptor antagonists can reduce the overall cerebral ischemia-reperfusion injury of Mongolian Geville by down-regulating autophagy in the cerebral cortex and hippocampus.