动物造模,记忆障碍 z-bio 2021-07-22 909

　　OBJECTIVE: To establish a memory disorder model of scopolamine and use traditional Chinese medicine compound Jin Shen to observe the effect of Jin Shen on the behavior and cholinergic system of mice with memory impairment caused by scopolamine.

　　Method: ICR mice were randomly divided into normal control group; model group, solvent 0.5?C; positive control group, donepezil, 0.92mg/(kg?d); Kings Vader? In the low-dose group, 20? 10? 5 mg/(kg? D)? Did the 18 mice in each group receive 0.1 mL/10 g mouse body weight for 30 consecutive days? After the last administration, the model was created. The control group was injected with normal saline, and the other groups were injected with scopolamine 3 mg/(kg?d) and 0.9? saline. The Morris water maze experiment was performed by injecting it to a body weight of 0.1 mL/10 g. Of mice. After the experiment, the cortex and hippocampus were collected, and the acetylcholine (Ach) content in the cortex and hippocampus were measured. What are the activities of esterase (AchE) and choline acetyltransferase (ChAT)?

　　Result: Golden thinking increases the residence time of model mice and target quadrants; can golden thinking increase Ach content in the brains of model mice? AchE activity decreases while ChAT activity increases?

　　Conclusion: Jinsiwei can improve the learning and memory ability of mice with memory impairment caused by scopolamine. Could this mechanism be related to the cholinergic system?